阙祖俊,董昌盛,罗斌,田建辉,等.人源肺癌循环肿瘤细胞系在不同品系小鼠上肺转移能力研究[J].,2018,5(5):26-28.
人源肺癌循环肿瘤细胞系在不同品系小鼠上肺转移能力研究
Study on lung metastasis of human lung cancer circulating tumor cell lines in different strains of mice
投稿时间:2018-03-19  最后修改时间:2018-04-12
DOI:10.12095/j.issn.2095-6894.2018.05.006
中文关键词:  循环肿瘤细胞;肺癌;转移;动物模型;
英文关键词:circulating tumor cell; lung cancer; metastasis; animal model
基金项目:国家自然科学基金面上项目(81373621);上海市市级医疗卫生优秀学科带头人培养计划(2017BR044);上海市青年科技英才扬帆计划(17YF1419700);龙医学者-育苗计划(LYTD-39)。
作者单位E-mail
阙祖俊 上海中医药大学附属龙华医院 030110371@mail.ecust.edu.cn 
董昌盛 上海中医药大学附属龙华医院  
罗斌 上海中医药大学附属龙华医院  
田建辉 上海中医药大学附属龙华医院 hawk7150@hotmail.com 
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中文摘要:
      目的: 探讨一种尾静脉注射人源肺癌循环肿瘤细胞建立的转移模型,比较NOD/ SCID 小鼠、Nude 小鼠和C57BL/6 小   鼠对该模型的敏感性。方法:将人源肺癌循环肿瘤细胞通过尾静脉注射接种到C57BL/6 小鼠、Nude 小鼠和NOD/ SCID 小鼠体   内,在不同时间节点对不同品系小鼠上的肺转移情况进行评估;H&E 染色检测肺组织病理变化。结果:CTC?TJH?01 细胞接种于   NOD/ SCID 小鼠体内后,在第8 周首次观察到肺部成瘤,在第10 周小鼠肺部成瘤率为100%。而在Nude 小鼠和C57BL/6 小鼠体   内均不成瘤。结论:人源肺癌循环肿瘤细胞通过尾静脉注射接种于NOD/ SCID 小鼠后能够发生肺转移,该模型可用于临床抗转   移药物筛选与评价研究
英文摘要:
      【Abstract】Objective: To investigate a metastasis model established by tail vein injection of human lung cancer circulating tumor cells,   and compare the sensitivity of NOD/ SCID mice, nude mice and C57BL/6 mice to this model. Methods:Human lung cancer circulating   tumor cells were inoculated into C57BL/6 mice, Nude mice, and NOD/ SCID mice via tail vein injection. The lung metastases of different   strains of mice were assessed at different time points; H&E staining was used to detect the pathological changes of lung tissue. Results:   After CTC?TJH?01 cells were inoculated into NOD/ SCID mice, the lung tumors were observed for the first time at the 8th week. In the 10th   week, the rate of tumor formation in the lungs of mice was 100%. However, tumors were not formed in Nude mice and C57BL/6 mice. Conclu?   sion: Human lung cancer circulating tumor cells can invade lung metastases after inoculation in NOD/ SCID mice by tail vein injection.   This model can be used for the screening and evaluation of clinical anti?metastatic
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